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Analytical method of 2-bromo-3-nitrobenzoic acid

Word:[Big][Middle][Small] QR Code 2019/11/27     Viewed:    

2-bromo-3-nitrobenzoic acid was analyzed by liquid phase method
1. Apparatus and reagents:
High performance liquid chromatography (HPLC), analytical balance, methanol chromatographic purity
2. Operation method:
Accurately weigh about 0.1g of the product, add 1ml methanol for dilution, and shake well.
3. Analysis conditions:
Column: 5um, C18, 4.6 * 150mm
Protective column cn
Flow rate: 2.0ml/min
Wavelength: 214nm
Injection volume: 0.2ul
Time: 15min
Mobile phase:
A: 0.1% trifluoroacetic acid aqueous solution
B: 0.1% trifluoroacetic acid acetonitrile solution
Analytical method of 2-bromo-3-nitrobenzoic acid


4. Normalization of calculation area, 98%+

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